In order to improve the production efficiency of camshaft, the facility layout of traditional camshaft production line was optimized based on genetic algorithm. Taking the lowest logistics cost of camshaft production line as the objective function, the total length, width and the minimum distance between equipments as the constraints, the mathematical model of camshaft production line was established, and the optimal solution was obtained by using MATLAB software. According to the obtained layout solution, the machine tool layout model of camshaft workshop was simulated by using Flexsim software. The results show that the daily output of camshaft increases obviously, the logistics cost is decreases after the facility layout is optimized, and the expected target is achieved.