In order to meet the demands of disorderly sorting and positioning of materials in the manufacturing process, an operation target location system of cooperative robots based on machine vision was designed.Qt was used to develop human-computer interaction interface to realize the functions of image acquisition and processing, communication and control of robot motion. The image was processed by threshold segmentation to obtain the ideal working area. Through the calibration of robot vision system, the conversion between camera pixel coordinates and robot base coordinates was realized, and the workpiece pose recognition in robot coordinate system was obtained. A certain number of workpieces were randomly placed on the robot target grasping platform for test. The results show that the system has advantages in accuracy and speed.